What is it about?
Yeast proteins and human proteins are far apart in evolution. Here we use X-ray crystallography to show that 14-3-3 proteins are remarkably conserved between man and yeast.
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Why is it important?
Our paper shows that 14-3-3 proteins evolved millions years ago and their structure&function remained virtually the same underlying their importance in physiology.
Perspectives
We spend tremendous amount of time trying to crystallize the complex of full length proteins. At the end we had to use just a short peptide from the PI4KB lipid kinase to obtain diffraction quality crystals.
Evzen Boura
Institute of Organic Chemistry and Biochemistry
Read the Original
This page is a summary of: Crystal structures of a yeast 14-3-3 protein fromLachancea thermotoleransin the unliganded form and bound to a human lipid kinase PI4KB-derived peptide reveal high evolutionary conservation, Acta Crystallographica Section F Structural Biology Communications, October 2016, International Union of Crystallography,
DOI: 10.1107/s2053230x16015053.
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