What is it about?
We applied the programs SnB, SOLVE and SHELXD to find the positions of the 30 expected Se atoms in the structure of SeMet UDP-N-acetylglucosamine 2-epimerase.
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Why is it important?
Selenomethionine was introduced into the structure to allow MAD phasing. Native crystals of Thermus thermophilus UDP-N-acetylglucosamine 2-epimerase crystallize in the hexagonal space group P3121 with unit cell parameters: a = b = 70.773 Å, and c = 139.002 Å. The SeMet analog also crystallizes in the same hexagonal space group P3121, but with unit cell parameters a = b = 70.097 Å, and c = 282.96 Å. Data to 2.28 Å resolution were collected with an ADSC Quantum detector at the SPring-8 beamline BL38B1 using cryo-cooled crystals.
Perspectives
The diffraction intensities of the SeMet analog at four different wavelengths near the Se K-edge were used to solve the phase problem. The best Se positions were used for the initial phasing.
Dr Bagautdin Bagautdinov
Read the Original
This page is a summary of: Crystallographic studies ofThermus thermophilusUDP-N-acetylglucosamine 2-epimerase at 2.28 Å resolution, Acta Crystallographica Section A Foundations of Crystallography, August 2002, International Union of Crystallography,
DOI: 10.1107/s010876730208827x.
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