What is it about?
Most eukaryotic expression systems make use of host-cell nuclear transcriptional and post-transcriptional machineries. Here, we present the first generation of the chimeric cytoplasmic capping-prone phage polymerase (C3P3-G1) expression system developed by biological engineering, which generates capped and polyadenylated transcripts in host-cell cytoplasm by means of two components. First, an artificial single-unit chimeric enzyme made by fusing an mRNA capping enzyme and a DNA-dependent RNA polymerase. Second, specific DNA templates designed to operate with the C3P3-G1 enzyme, which encode for the transcripts and their artificial polyadenylation. This system, which can potentially be adapted to any in cellulo or in vivo eukaryotic expression applications, was optimized for transient expression in mammalian cells. C3P3-G1 shows promising results for protein production in Chinese Hamster Ovary (CHO-K1) cells. This work also provides avenues for enhancing the performances for next generation C3P3 systems.
Featured Image
Why is it important?
Expression rate is critical for most biological purposes. C3P3 technology outreaches the performances of classical expression technologies.
Perspectives
Used for a new type of gene therapy named synthetic gene therapy, as well as recombinant protein and virus bioproduction
Dr Philippe H Jais
philippe.jais@eukarys.com
Read the Original
This page is a summary of: C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system, Nucleic Acids Research, February 2019, Oxford University Press (OUP),
DOI: 10.1093/nar/gkz069.
You can read the full text:
Contributors
The following have contributed to this page
