What is it about?
This protocol describes a purification strategy that combines the in vitro enzymatic relaxation of sc MP and PP impurities by a nicking endonuclease, and topoisomer separation and RNA clearance by hydrophobic interaction chromatography.
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Why is it important?
A wider application of minicircle (MC) vectors in gene therapy research depends critically on the ability to purify supercoiled (sc) MC from related miniplasmid (MP) and parental plasmid (PP) impurities.
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This page is a summary of: Production and Purification of Supercoiled Minicircles by a Combination of in vitro Endonuclease Nicking and Hydrophobic Interaction Chromatography, Human Gene Therapy Methods, July 2018, Mary Ann Liebert Inc,
DOI: 10.1089/hgtb.2018.046.
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