3D printed absorbance measurement platform

What is it about?

By harnessing 3D printing technology, we have devised a compact handheld device designed using computer-aided design (CAD) and 3D printing, integrating SMD LED-photodiode for conducting MTT assays. The MTT solution containing varying concentrations of drugs was introduced into a microfluidic phase guide device, which is then inserted into the 3D-printed device, housing SMD devices and relevant electronic circuitry for absorbance measurement. Measurements are facilitated using a capacitive touchscreen display that can be linked to external devices for data transmission. The developed device efficiently displayed the total cell viability for each drug concentration. Experimental results were compared with those obtained using a standard spectrophotometer for MTT assay, revealing a high correlation coefficient (R2 = 0.98). This robust and refined approach offers a rapid, highly sensitive, and cost-effective means for conducting bioassays, holding promise for rapid and extensive commercialization.

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Photo by Vadim Bogulov on Unsplash

Photo by Vadim Bogulov on Unsplash

Why is it important?

Development of compact and affordable platforms towards performing biological assays is one of the primary interests especially after covid-19 era. Majority of the assays/diagnostics are done at clinical labs/hospitals using complex instruments thereby making it unaffordable, requirement of trained manpower and unreachable in undeveloped and developing countries. To combat this, we have developed a 3D printed platform which can seamlessly perform cell toxicity assay through absorbance measurement. Integration of Surface mount devices along with microfluidic device enabled a miniature platform which was compared with traditional spectrophotometer.

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