Guidelines for antibody validation

What is it about?

The European Monoclonal Antibody Network provides a simple practical step-by-step guide to enable researchers to find antibodies that can recognise their target proteins and then validate that they have the desired activity and specificity.

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Why is it important?

There is a huge demand for new antibodies. However, many research antibodies fail even very basic quality control testing, such as their ability to recognise their target protein. Researchers thus waste time and money testing these antibodies , while data obtained with poorly validated antibodies introduces errors into the scientific literature . Practical guidelines for choosing and validating antibodies will help even researchers with little experience use these reagents effectively.