What is it about?
This invited addendum highlights features of our study on maize DNA replication using 3D microscopy - also shows how to access hundreds of 3D image from our study, great for additional research or teaching cytogenetics.
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Why is it important?
The article addresses some interesting questions not explored in detail in the original paper (Bass et al. Plant Mol Biol; 2015), including comparative analysis of replication patterns in mammals versus maize, implications and predictions from the 2-domain chromatin model, and questions about replication inside the nucleolus. The article also announces a related teaching and research resource in the form of a 3D image server, open to the public, and available online with user name and password provided in the article. Unlike genomic data repositories, cell biology image data bases do not exist, resulting in a loss of access to raw data after publication, limiting further exploration. The server, at omero.bio.fsu.edu, features more than 300 2-color (total DNA vs replicating DNA) images sorted by stage (early, middle, or late S) and in two cell cycle contexts (mitotic or endocycle).
Perspectives
This article actually is our "author's perspective", an invited addendum to our 2015 PMB article. First author, Savannah Savadel, is an undergraduate research scholar at FSU, and premed biology major. She helped write the paper an organize and annotate the images in omero, most of which were originally collected by Gregg Hoffman. The entire project stems from a productive collaboration with colleagues (Bill Thompson, Linda Hanley-Bowdoin) at NCSU, the group pioneering state-of-the-art plant DNA replication research using naturally developing root tips. In fact, this aspect (studying replication in nuclei from naturally developing organism) sets it apart from the many volumes of data on DNA replication in mammals, most of which relies on cell culture systems.
Dr Hank W Bass
Florida State University
Read the Original
This page is a summary of: Take a look at plant DNA replication: Recent insights and new questions, Plant Signaling & Behavior, April 2017, Taylor & Francis,
DOI: 10.1080/15592324.2017.1311437.
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