What is it about?
Although transgenic chickpea has already been produced, the transformation rate was low due to its reluctance to regeneration. This paper describes an effective, rapid, and simple protocol for the regeneration of chickpea. In particular, we have shown a systematic comparison of the regeneration response of different types of explants to various types and concentrations of growth regulators in two Egyptian chickpea varieties.
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Why is it important?
Three significant findings are that: a) the suitability of all kind of explants derived from the embryonal axis for shoot organogenesis in chickpea, b) the ability of our explants to maintain and/or synthesize the required endogenous hormones and c) the explant type have not only a direct effect on shoot regeneration but also an indirect impact on root regeneration to some extent. This protocol provides an efficient mean for the regeneration of chickpea, as well a basis for further research aimed at genetic improvement of this plant.
Perspectives
I am very proud of this manuscript as it joins me (as a young researcher) with co-authors who are highly experienced. I also hope that the findings from this work can pave the way toward the genetic improvement of the Egyptian chickpea.
Dr. Ahmed M. Amer
National Research Centre
Read the Original
This page is a summary of: In vitro regeneration through organogenesis in Egyptian chickpea, Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology, January 2019, Taylor & Francis,
DOI: 10.1080/11263504.2018.1549616.
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