Flavonoids determination

What is it about?

The scheme of postcolumn complexation reaction of flavonoids with aluminum (III) ions in various microemulsion media followed by fluorimetric detection is proposed. It is shown that fluorescence intensity of such complexes is five, three, and three times higher for quercetin, isorhamnetin, and kaempferol, respectively, when compared with the complexes obtained by dissolving flavonoids in MeCN/0.1 M HCl (30/70) and AlCl3. The prospect of using microemulsions for quantitative quercetin extraction from plant raw material is demonstrated. Recovery of quercetin from onion peel is 98% with detection limits being 100, 45, and 15 ng/L for quercetin, isorhamnetin, and kaempferol, respectively. Quercetin content in three types of onion peel is determined. This content is found to be 34 +-3 and 5 +-1 mg/g for yellow and purple onion peel, respectively, while the content in white onion peel is below the detection limit.

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