What is it about?
We have developed a modeling strategy that accurately mimics human lung cancer. Lung cancer development involves multiple genetic alterations, so there is a need for a gene editing system that can rapidly generate sets of mutations. Using CRISPR-Cas9 technology, we made DNA modifications targeting tumor suppressor genes. We used synthetic biocompatible materials to deliver CRISPR-Cas9 directly to airway cells. This approach allowed us to develop a small-cell lung cancer model that closely mimics the characteristics of the human disease, which is essential for studying and testing treatments.
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Why is it important?
This innovative strategy offers a swift, cost-efficient avenue for cancer modeling, which is crucial for functional genomics research.
Perspectives
In vivo non-viral gene editing in tissues using CRISPR delivery will facilitate the flexible generation of complex models that reflect the complexity of cancer.
Mirentxu Santos
Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas (CIEMAT)
Read the Original
This page is a summary of: Nonviral CRISPR/Cas9 mutagenesis for streamlined generation of mouse lung cancer models, Proceedings of the National Academy of Sciences, July 2024, Proceedings of the National Academy of Sciences,
DOI: 10.1073/pnas.2322917121.
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