What is it about?
Here, we embedded two counter-propagating optical fibers into the microfluidic chip, and configured their relative position in space to produce different misalignment. By doing so, we demonstrated multimodal manipulation of single cells, including capture, stretching, translation, orbital revolution, and spin rotation.
Featured Image
Photo by Mimicry Hu on Unsplash
Why is it important?
Single cell manipulation is imperative in biotechnology to facilitate many potential applications, such as cell injection, imaging, positioning, and electrical properties characterization. Basic manipulation includes translation and rotation of cells, among many others.
Perspectives
We successfully reconstructed the 3D morphology of single cells from a stack of images from out-of-plane spine rotation and calculated their physical parameters from the 3D image. This 3D imaging technique provides a possible alternative to confocal microscopy with no need of labeling, as a new tool for cell characterization. The dual-fiber microfluidic chips can efficiently realize single cell multimodal manipulation, and provide a convenient platform for single cell research.
Professor Wenhui Wang
Tsinghua University
Read the Original
This page is a summary of: Dual-fiber microfluidic chip for multimodal manipulation of single cells, Biomicrofluidics, January 2021, American Institute of Physics,
DOI: 10.1063/5.0039087.
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