What is it about?
The broad-host-range plasmid pAMbeta1 of Gram-positive bacteria initiates replication by a D-loop structure formed by the DNA polymerase I-mediated strand displacement activity. Here we show that the pAMbeta1 encoded topoisomerase as a type I topoisomerase activity that promotes premature arrest of DNA polymerase I about 190 bp downstream of the initiation site of replication.
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Why is it important?
The premature arrest of DNA polymerase I by the topoisomerase (and by a resolvase-DNA complex, see paper published in Mol Mic 2007) generates a small D-loop that contains a primosome assembly site. This arrest thus assists the switch from DNA Pol I to the replisome and thus improves the fidelity of plasmid replication. This mechanism likely applies to ColE1-type plasmids of Escherichia coli.
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This page is a summary of: In vivo relations between pAM?1-encoded type I topoisomerase and plasmid replication, Molecular Microbiology, June 1998, Wiley,
DOI: 10.1046/j.1365-2958.1998.00862.x.
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