What is it about?
In this article, we report a dielectrophoresis-based, on-chip manipulation method that can load and rotate single cell for 3D cell imaging and multiple biophysical property measurement.
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Why is it important?
3D rotation is one of many fundamental manipulations to cells and imperative in a wide range of applications in single cell analysis involving biology, chemistry, physics and medicine. Using our device, we quantified the area-specific membrane capacitance and cytoplasm conductivity for the four cells, and revealed the subtle difference of geometric parameters (i.e., surface area, volume, and roughness) by 3D cell imaging for cancer cells and normal leukocytes.
Perspectives
Combining microfluidics, dielectrophoresis, and microscopic imaging techniques, our electrorotation on chip (EOC) technique is a versatile method for manipulating single cells under investigation and measuring their multiple biophysical properties.
Professor Wenhui Wang
Tsinghua University
Read the Original
This page is a summary of: 3D cell electrorotation and imaging for measuring multiple cellular biophysical properties, Lab on a Chip, January 2018, Royal Society of Chemistry,
DOI: 10.1039/c8lc00407b.
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