Quality assessment of recombinant proteins by infrared spectroscopy. Characterisation of a protein aggregation related band of the Ca2+-ATPase

What is it about?

A feature in the infrared spectrum of recombinant Ca2+-ATPase could be attributed to aggregated protein. This explains the lower activity of the recombinant protein as compared to the rabbit muscle preparation. It provides a simple monitor for improving the purification protocol for the ongoing study of Ca2+-ATPase mutants.

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Why is it important?

Infrared spectroscopy provides a simple monitor to detect aggregation of recombinantly produced protein. This can be used to improve the purification protocol.