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One of the holy grails of ligation is a three piece ligation without the need for further protecting groups. Here is one approach to that with an auxiliary mediated ligation in the presence of unprotected cysteine.

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This page is a summary of: Orthogonal ligation: a three piece assembly of a PNA–peptide–PNA conjugate, Chemical Communications, January 2008, Royal Society of Chemistry,
DOI: 10.1039/b801242c.
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