What is it about?
This paper presents a lateral flow assay (LFA) for the quantitative, fluorescence-based detection of the kidney biomarker cystatin C that features conjugates of capture antibodies and fusions of carbohydrate binding modules (CBM) with ZZ domains anchored on cellulose deposited over nitrocellulose (NC). The ZZ-CBM3 fusion provides a biomolecular interface between the cellulose layer and the Fc portion of the capture antibodies. By resorting to detection Fab fragments that lack the Fc portion we overcome the observed interference of full-length detection antibodies with the ZZ-CBM3 fusion at the test lines.
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Why is it important?
The current relevance and impact of LFA in the diagnostics arena could significantly expand if drawbacks like low sensitivity, low specificity, and lack of quantitation can be overcome. Significant research efforts are thus being devoted to bringing the overall performance of LFAs close to that afforded by standard laboratory tests like ELISA and PCR.
Read the Original
This page is a summary of: Exploring carbohydrate binding module fusions and Fab fragments in a cellulose-based lateral flow immunoassay for detection of cystatin C, Scientific Reports, March 2022, Springer Science + Business Media,
DOI: 10.1038/s41598-022-09454-9.
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Resources
News of the project CBM-X: Biorecognition as a Tool for the Functionalization of Cellulose-based Materials
Collection of short news related to the project "CBM-X: Biorecognition as a Tool for the Functionalization of Cellulose-based Materials with Biomolecules and Nanostructures", funded by FCT (2017 Call for SR&TD Project Grants). The goal of CBM-X is to develop biomolecular constructs for the precise modification of cellulose matrices with nanostructures and/or biomolecules, and for the anchoring of nanocellulose onto a range of materials.
Open access version of the paper
Full version of the paper
Biorecognition as a tool for the functionalization of cellulose
A brief overview of my research on the use of carbohydrate binding modules as a tool for the functionalization of cellulose-based materials.
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