What is it about?
An imaging-based method for analysis of antibiotics actions including modified-vancomycins
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Why is it important?
Whole cell-based imaging assays could compensate for the drawbacks of in vitro enzyme-based assay. For example, minimum inhibitory concentration (MIC) is an essential criterion for screening large compound libraries or microbial extracts in the pursuit of new antibacterial agents, but it does not provide insight into the modes of action. This assay can provide quick feedbacks on the mode of action of antibiotic-lead just prepared. Thus, it accelerates medicinal chemistry research.
Perspectives
In ovococcal bacteria, two type of cell-wall synthesis exist, which are peripheral and septal syntheses. We found that some antibiotics suppress specifically the septal synthesis but not the other. The differences of peripheral and septal syntheses are not well understood to date. This may be an important clue for development of antibacterial agents with novel actions.
Prof Hirokazu Arimoto
Tohoku Daigaku
Read the Original
This page is a summary of: Deciphering the mode of action of cell wall-inhibiting antibiotics using metabolic labeling of growing peptidoglycan in Streptococcus pyogenes
, Scientific Reports, April 2017, Nature,
DOI: 10.1038/s41598-017-01267-5.
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Contributors
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