What is it about?
A report on Nicotiana africana protoplast isolated from cell suspension culture and plant regeneration.Plant protoplast are cells without cell walls and they can regenerate in vitro to whole plants. We were able to demonstrate regeneration from cell suspension protoplast in a new tobacco species Nicotiana africana.
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Why is it important?
Protoplast culture and plant regeneration is important to transfer resistance traits, like resistance to potato virus Y (PVY) by fusing protoplast of two different species, into cultivated species like N. tobacco or potato (this was not successful because of difficulties in regenerating fusion products between the two genus). It is also important to use protoplast for gene transfer or today for genome editing with CRISPR-Cas technology.
Perspectives
Since the Nicotiana tabacum is a model species for in vitro culture of plant protoplast the regeneration of N. africana protoplast opens the way for somatic hybridization and genetic transformation.
Professor Elena I Rakosy-Tican
Universitatea Babeş-Bolyai Cluj-Napoca
Read the Original
This page is a summary of: , Plant Cell Tissue and Organ Culture (PCTOC), January 1998, Springer Science + Business Media,
DOI: 10.1023/a:1006129723477.
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