What is it about?
Triggering protein reactions is often difficult in infrared spectroscopic experiments. Here, a method to induce consecutive phosphate transfer reactions is described. It is used to characterise how the structure of a β-sheet in the Ca2+-ATPase is modified by binding of ATP's γ-phosphate.
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Why is it important?
This work makes infrared spectroscopy applicable to the study of more enzyme reactions.
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This page is a summary of: Use of Creatine Kinase To Induce Multistep Reactions in Infrared Spectroscopic Experiments, The Journal of Physical Chemistry B, November 2013, American Chemical Society (ACS),
DOI: 10.1021/jp409599p.
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