Structure of PA1221, a Nonribosomal Peptide Synthetase Containing Adenylation and Peptidyl Carrier Protein Domains

What is it about?

The Structure of PA1221, a Nonribosomal Peptide Synthetase Containing Adenylation and Peptidyl Carrier Protein Domains biochemically and structurally characterizes an adenylation:Peptidyl Carrier Protein di-domain Nonribosomal Peptide Synthetase. The use of a vinyl sulfonamide mechanism-based inhibitor was necessary to trap the two domains in a biologically relevant pose allowing the characterization of the interactions between the two domains.

Featured Image

Why is it important?

The Structure of PA1221, a Nonribosomal Peptide Synthetase Containing Adenylation and Peptidyl Carrier Protein Domains validated the engineered di-domain structure available at the time. The interactions are more or less conserved between the two structures. Furthermore, the use of the mechanism based inhibitor was necessary to get the holo-crystal form. Biochemical studies supported that the di-domain is capable of inter and intramolecualr transfer to the PCP domain.