Structure–Function Assessment of Mannosylated Poly(β-amino esters) upon Targeted Antigen Presenting Cell Gene Delivery

What is it about?

The manuscript describes a systematic evaluation of gene delivery to antigen presenting cells (APCs). In particular, the chemical background of a poly(beta-amino acid) was maintained constant to evaluate the impact molecular weight, mannosylation, and accompanying biophysical properties had upon gene delivery.

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Why is it important?

Our results demonstrate potent gene delivery as a by-product of the molecular parameters engineered in this study. Namely, gene delivery surpassed levels achieved with commercial transfection agents. Furthermore, the trends associated with structural and biophysical properties of the synthesized vectors indicate paradigm-contradicting results and suggest new design heuristics for APC gene delivery. Such insight is thus particularly useful in genetic vaccine strategies focused on antigen delivery to macrophage and dendritic cell APCs. As such, we believe the data and results presented will resonate with a wide range of basic and applied scientists across the disciplines of polymer chemistry, biomaterial vector formulation, and immunotherapy applications.