What is it about?
The article describes a method to detect pathogen DNA using bioactive paper strips.
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Why is it important?
DNA tests on paper are affordable, easy to perform by non-specialised personnel and do not require expensive equipment. These characteristics will make it possible to perform diagnostic of infectious disease in low-resource settings.
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This page is a summary of: Capture and Detection of DNA Hybrids on Paper via the Anchoring of Antibodies with Fusions of Carbohydrate Binding Modules and ZZ-Domains, Analytical Chemistry, April 2014, American Chemical Society (ACS),
DOI: 10.1021/ac5001288.
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Binding of a carbohydrate binding module fused to green fluorescent protein to cellulose microparticles
Fluorescence microscopy photography of carbohydrate binding module-green fluorescent protein (CBM3-GFP) fusions bound to cellulose 20 micron-sized microparticles. The CBM3 is from C. thermocellum.
Microscopic structure of chromatographic paper (700X)
Scanning electron microscope image of chromatographic paper Whatman N. 1 showing the tangle of mulitscale fibers and network of pores characteristic of cellulose. The paper sample was covered with a conductive layer of gold-palladium particles and observed on a Hitachi S2400 SEM microscope in the low vacuum mode.Magnification: 700X.
Microscopic structure of wax-printed chromatographic paper (700X)
Scanning electron microscope image of wax-printed chromatographic paper Whatman N. 1. A relatively smooth surface layer of wax can be seen in the printed portion of the paper on the right. A non-printed portion of the paper is seen on the left side, with its characteristic tangle of mulitscale fibers and network of pores. The paper was printed with a Xerox ColorQube 8570 wax printer. A sample was then covered with a conductive layer of gold-palladium particles and observed on a Hitachi S2400 SEM microscope in the low vacuum mode. Magnification: 700X.
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