What is it about?
In IFC, the uncertainty of the cell's spatial position in the microchannel will bring measurement errors in single-cell electrical parameters. To address the issue, we designed a novel microdevice configured with a coplanar differential electrodes structure to accurately resolve the spatial position of single cells without constraining techniques such as additional sheath fluids or narrow microchannel.
Featured Image
Photo by Denny Müller on Unsplash
Why is it important?
The device precisely localizes single cells by measuring the induced current generated by the combined action of the floating electrode and the differential electrodes when single cells flow through the electrode sensing area. The device was experimentally validated by measuring 6 μm yeast cells and 10 μm particles, achieving spatial localization with a resolution down to 4 μm (10% of the channel width) in lateral direction and 5 μm (20% of the channel height)
Perspectives
The device offers a competitive electrode configuration in impedance cytometry with the advantages of simple structure, low cost and high throughput, promising accurate cell localization and thus electrical characterization.
Professor Wenhui Wang
Tsinghua University
Read the Original
This page is a summary of: Floating-Electrode-Enabled Impedance Cytometry for Single-Cell 3D Localization, Analytical Chemistry, March 2023, American Chemical Society (ACS),
DOI: 10.1021/acs.analchem.2c05822.
You can read the full text:
Contributors
The following have contributed to this page
