What is it about?
Here, we present a streamlined strategy for highly efficient and automatic single-cell multi-omics analysis by MS, featuring 5-min in-fly protein digestion in 10-pL-level microwells and automated extraction ESI system.
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Photo by Andrei Zolotarev on Unsplash
Why is it important?
The efficient analysis of multi-omics in single cells, however, is still challenging in the manipulation of single cells and lack of in-fly cellular digestion and extraction approach. This strategy could facilitate the detection of 1391 proteins, phospholipids, and metabolites from one single cell within 10 min.
Perspectives
We analyzed cells digested from cancer tissue samples, achieving up to 40% increase in cell classification accuracy using multi-omics analysis in comparison with single-omics analysis. This automated single-cell MS strategy is highly efficient in ana-lyzing multi-omics information for investigation of cell heterogeneity and phenotyping for biomedical applications.
Professor Wenhui Wang
Tsinghua University
Read the Original
This page is a summary of: Efficient Sample Preparation System for Multi-Omics Analysis via Single Cell Mass Spectrometry, Analytical Chemistry, April 2023, American Chemical Society (ACS),
DOI: 10.1021/acs.analchem.2c05728.
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