What is it about?
This method has a powerful detection unit, based on thermal lens spectroscopy. Thus, it can analyze both bilirubin and biliverdin in biological samples at very low concentration. This means that the sample preparation is limited, which preserves the molecules to analyze.
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Why is it important?
This method has enabled the first-ever detection of biliverdin in human serum (ca. 2 nM) and first-ever assessement of bilirubin (free)/biliverdin ratio in human serum (ca. 2-4).
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This page is a summary of: Application of high-performance liquid chromatography combined with ultra-sensitive thermal lens spectrometric detection for simultaneous biliverdin and bilirubin assessment at trace levels in human serum, Talanta, July 2016, Elsevier,
DOI: 10.1016/j.talanta.2016.03.053.
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