What is it about?
Effects of phytol on methotrexate-induced folate deficiency (Anemia). We showed the protective effects of phytol and mechanisms of how phytol increases RBC in Swiss albino mice resulting in attenuation of anemia. For this, a total of thirty adult male Swiss albino mice were randomly divided into six different groups, namely the normal control (vehicle), the negative control (folate deficiency inducer, methotrexate 3 mg/kg), the standard (folic acid 1.5 mg/kg), two test groups comprising phytol 25 and 50 mg/kg, and a combined group composed of the standard and highest test doses of phytol. Except for the vehicle, all groups were treated with methotrexate for the first 3 days (once/day) to induce folate deficiency. Then followed by the respective treatment once a day for 3 days. Hemoglobin (Hb) level was measured from the peripheral blood (by tail cutting) on days 1st (before treatment), 4th (after methotrexate treatment), and 7th (after treatment). On the other hand, the computational studies were performed by PyMol, PyRex, Discovery Studio, and other complementary tools. Findings suggest that phytol significantly (p <0.05) augmented Hb levels that are altered by methotrexate-induced reduction of Hb levels in animals dose-dependently. The combination also augmented Hb levels in animals; however, its effect was slightly lower than the individual groups (standard and test). In the in silico study, phytol showed good binding capacity (binding energy: −7.0 kcal/mol) with dihydrofolate reductase (DHFR). In conclusion, phytol may act against folate deficiency by altering methotrexate’s impacts in animals, possibly through interacting with DHFR. Further validated research is necessary to develop phytol as an anti-anemia drug in the near future.
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Why is it important?
Phytol may act against folate deficiency by altering methotrexate’s impacts in animals, possibly through interacting with DHFR. Further validated research is necessary to develop phytol as an anti-anemia drug in the near future.
Perspectives
The findings of our study indicated that phytol significantly enhanced Hb in MTX-induced folate-deficient Swiss albino mice compared to the NC and NgC groups
Md Shimul Bhuia
Bioinformatics and Drug Innovation Laboratory, BioLuster Research Center
Read the Original
This page is a summary of: Possible hemoglobin enhancing effect of phytol in methotrexate-induced folate deficient Swiss albino mice: In vivo and in silico studies, Pharmaceutical Science Advances, December 2024, Elsevier,
DOI: 10.1016/j.pscia.2024.100043.
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