What is it about?
Recently introduced megaporous cryogels are relatively new and have not been fully exploited for the downstream processing of macro-biomolecules. Weak anion-exchange functionality was incorporated by gamma irradiation-induced grafting. The total ionic capacity obtained for DEAE-functionalized cryogel was 0.59 meq/g. Scanning electron microscopy confirmed that the porous nature of the cryogels remained unaffected by the irradiation-grafting procedure.
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Why is it important?
The resulting weak anion-exchange cryogels showed a pore size distribution of up to 100 μm with dynamic binding capacities of ca. 27 ± 3 mg/mL. The dynamic binding capacities of monolithic cryogels were found to be independent of the applied flow rate. Due to the large pores, high permeability, disposability, and cost-efficient nature, megaporous cryogels are an attractive alternative for the purification of macro-biomolecules like mAbs, mRNA, pDNA and viruses.
Perspectives
The adsorbents presented in here showed better column efficiency when compared to packed-bed adsorbents and also had better protein binding capacities.
Dr Naveen Kumar Singh
University of Notre Dame
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This page is a summary of: Gamma ray-mediated functionalization of monolithic cryogels for macro-biomolecule purification, New Biotechnology, July 2014, Elsevier,
DOI: 10.1016/j.nbt.2014.05.1924.
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