What is it about?
It is about making modifications in a phenol/chloroform extraction method and comparing it to a commercial kit.
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Why is it important?
Using commercial kits, Extracting bacterial DNA from feces of horses is challenging due to low quality and quantity of the recovered DNA specially the high presence of the inhibitors to the downstream applications such as polymerase chain reaction (PCR) and next generation sequencing techniques. Here, our modifications in a phenol/chloroform method have removed these challenges. It gives high quality and quantity of the extracted DNA.
Perspectives
Using commercial kits, Extracting bacterial DNA from feces of horses is challenging due to low quality and quantity of the recovered DNA specially the high presence of the inhibitors to the downstream applications such as polymerase chain reaction (PCR) and next generation sequencing techniques. Here, our modifications in a phenol/chloroform method have removed these challenges. It gives high quality and quantity of the extracted DNA.
Assist. Prof. Dr. Ali H.D. Janabi
University of Al-Qadisiyah
Read the Original
This page is a summary of: Comparison of a modified phenol/chloroform and commercial-kit methods for extracting DNA from horse fecal material, Journal of Microbiological Methods, October 2016, Elsevier,
DOI: 10.1016/j.mimet.2016.07.019.
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