What is it about?
Absolute quantification of tear proteins is beneficial for eye research, since the tear proteins reflect the health of the eye and various proteins are deranged in different diseases. The diseases may range from common ones such as dry eye, chemical injury and allergies, to devastating infections and scarring conditions. Despite the usefulness of this approach, absolute quantification of proteins is still challenging, so we evaluate one possible strategy for 47 proteins commonly encountered.
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Why is it important?
It is now possible to compare absolute protein levels across different people or between different samples of tears collected at different time points. This will allow monitoring of patients on a very objective basis, as well as clinical trials to be conducted with objective outcomes.
Perspectives
The tear proteome is indeed a good reflection of the health of the extracellular matrix in the ocular surface.
Prof Louis Tong
National University of Singapore
Read the Original
This page is a summary of: Quantitation of 47 human tear proteins using high resolution multiple reaction monitoring (HR-MRM) based-mass spectrometry, Journal of Proteomics, February 2015, Elsevier,
DOI: 10.1016/j.jprot.2014.12.002.
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