Identification of Xanthomonas campestris transcription start sites by RNA-seq

What is it about?

RNA-seq has been applied to Xanthomonas campestris pv. campestris, a model organism for pant pathogenic bacteria and producer of the polysaccharide xanthan, to globally determine transcription start sites. This is the first such analysis for a bacterium of the genus Xanthomonas.

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Why is it important?

Knowledge of transcription start sites greatly facilitates systematic recognition of promoter sites, which are crucial for the regulation of gene expression. This data is a valuable basis that will facilitate the analysis of regulatory processes in Xanthomonas bacteria, where such regulation is so far only poorly conceived.