What is it about?
Deep eutectic solvents (DESs) are promising for biocatalysis due to their tunability and protein-stabilizing properties. We explored solvation dynamics and stability of human serum albumin (HSA) in 0.5Ac/0.3Ur/0.2Sor DES. Our findings show that beyond 30% DES, HSA's side-chain flexibility and thermal stability decrease, while water dynamics slow only marginally with increased viscosity.
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Why is it important?
Protein are most efficient biocatalyst, but the difficulty is proteins are only stable and active in water. One way out is to create mutated enzymes which remain stable and active in organic solvent. Easier way could be preparing solvent in which protein could be stable and active. In recent time, ionic liquids and deep eutectic solvent has emerged as such solvents. However, any research field truely progresses only when there is a proper physical insight. Through our work, we suggested that proteins experience an aqueous-like environment in DES, providing insight into their stability and activity in such media.
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This page is a summary of: Understanding the intricacy of protein in hydrated deep eutectic solvent: Solvation dynamics, conformational fluctuation dynamics, and stability, International Journal of Biological Macromolecules, December 2023, Elsevier,
DOI: 10.1016/j.ijbiomac.2023.127100.
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