What is it about?

The aim of our study was thus to investigate the digestion and absorption characteristics of triacylglyceride monomers from SPO. In this study, Pre-LC was used for the first time to separate triacylglyceride monomers. The separation scheme of Pre-LC was optimized regarding chromatographic peak shape and preparation time, and product structure was identified by HPLC-ELSD, 1H nuclear magnetic resonance (1H NMR) and MS. The pH-stat system and Caco-2 cells model evaluated the connections among triacylglycerol composition and lipid gastrointestinal digestive absorption profiles. Finally, the regulation of lipid metabolism genes was clarified based on the Caco-2 cell model. In general, the nutritional value of the triacylglyceride monomers in SPO was evaluated through the integrated digestion and absorption process, which clarifies the regulation of its lipid metabolism related genes.

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Why is it important?

In the present study, Pre-LC was used to separate SPO for the first time, and pH-stat in vitro digestion model and Caco-2 cell model were constructed to investigate the in vitro digestion and absorption characteristics of SPO and its triacylglyceride monomers. Under the optimal preparation conditions, two triacylglyceride monomers were successfully isolated, namely LnLnLn and LnLnL

Perspectives

mRNA expression levels of lipid metabolism-related proteins provide evidence for the absorption superiority of LnLnLn and LnLnL. The present work provides novel structural lipids for rapid and efficient supplementation of α-linolenic acid.

Dr Richard Ansah Herman
Jiangsu University of Science and Technology

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This page is a summary of: Gastrointestinal digestion fates of lipids based on pH-stat and Caco-2 cells models: Superior bioavailability of triglyceride monomer derived from silkworm pupae oil, Food Bioscience, June 2023, Elsevier,
DOI: 10.1016/j.fbio.2023.102700.
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