What is it about?
Rhenium (I) complexes can be used as staining probes for non-walled cells. Less is known regarding their use for walled cells (yeasts and bacteria). In this study, we synthesized and characterized ReL1 and ReL2. ReL2 exhibited good photophysical features as fluorophore. ReL2 was useful to stain yeasts and bacteria for confocal microscopy.
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Why is it important?
We also assessed the cytotoxicity of ReL1, ReL2, and their respective N,N ligands (i.e., 4,5-diazafluoren-9-one and 5,6-epoxy-5,6-dihydro-1,10-phenanthroline) against walled-cells, including Gram-negative bacteria (Salmonella enterica serovar Typhimurium), non-sporulated Gram-positive bacteria (Staphylococcus aureus), sporulated Gram-positive bacteria (Bacillus cereus), and yeasts (Candida albicans and Cryptococcus spp.). We observed that these complexes exhibited very low or no cytotoxicity. We also found that only ReL2, and not ReL1, exhibited good properties as a luminescent probe for this kind of cells.
Perspectives
We found that ReL2 showed good potential to be directly used as a luminescent probe for walled cells, including yeasts (e.g., Candida albicans) and bacteria (e.g., Salmonella enterica), which can be observed by confocal microscopy.
Dr Alexander Carreño
UNAB
Read the Original
This page is a summary of: Exploring rhenium (I) complexes as potential fluorophores for walled-cells (yeasts and bacteria): Photophysics, biocompatibility, and confocal microscopy, Dyes and Pigments, September 2020, Elsevier,
DOI: 10.1016/j.dyepig.2020.108876.
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