What is it about?
A new process is devised to purify minicircles, a special type of DNA molecules that can be used in gene therapy applications. Specifically, we resort to an enzyme to modify the topology of a key impurity and subsequently use a chromatographic separation to isolate minicircles.
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Why is it important?
Highly pure minircles must be available for developing gene therapy applications.
Perspectives
A tough separation accomplished with the aid of an enzyme.
Professor Duarte Miguel Prazeres
Instituto Superior Técnico
Read the Original
This page is a summary of: Development of a nicking endonuclease-assisted method for the purification of minicircles, Journal of Chromatography A, April 2016, Elsevier,
DOI: 10.1016/j.chroma.2016.03.035.
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