What is it about?
We developed a method that exploited the difference in the velocity of the enzymes' glycoforms in the presence of an electric field to separate them before reaching the detection region. This is useful fir determining the variations in the relative concentration of the glycoforms in different samples.
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Why is it important?
The enzyme studied in this work is important for the conversion of "woody material" to biofuel. The enzyme's activity is strongly influenced by the relative distribution of its glycoforms. The information obtained by the developed method on the microheterogeneities in glycoforms is, therefore, useful for adapting the feed ratio of enzyme samples to improve the efficiency of bioethanol production.
Perspectives
The enzyme would have been produced by genetically modified sugarcane. A challenging aspect was to be able to measure the relative concentration distribution of an enzyme's glycoforms in sugarcane juice.
Dr Ruchi Gupta
University of Birmingham
Read the Original
This page is a summary of: Capillary zone electrophoresis for the analysis of glycoforms of cellobiohydrolase, Journal of Chromatography A, August 2011, Elsevier,
DOI: 10.1016/j.chroma.2011.06.036.
You can read the full text:
Resources
Electropherogram showing separation of glycoforms of the enzyme
The glycoforms differ primarily in the number of a simple sugar, mannose, present in oligosaccharides attached to the enzyme
Video on Biofuels
Introduce biofuel production from "woody" material e.g. corn stalks
E10 - Fuel with 10% bioethanol
Highlights some of the benefits of using E10 to run our vehicles
Enogen - corn enzyme technology for biofuel production
Alpha amylase is expressed in corn kernels to eliminate the need to add the enzyme externally for producing bioethanol from corn
Contributors
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