Effect of Temperature on Parameters of Fluorescence Spectra of Solvatochromic Indicator Solutions in Stationary Phases for Gas–Liquid Chromatography

What is it about?

Luminescence spectra of several polarity indicators, including Coumarin 153 and coronene that were previously studied at room temperature and Nile Red and Pigment Red 179, were recorded at 20 and 120oC in solutions of liquid stationary phases of various polarities. The eff ect of temperature on the spectral parameters sensitive to the stationary phase polarity was estimated for these indicators. The prospects for estimating the polarity of stationary liquid phases using luminescent methods at high temperatures was demonstrated.

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Why is it important?

In this work the polarity of stationary phases is evaluated by using fluorescence of polarity indicators. The polarity of GC stationary phases is varied with temperature and the fluorescence can help to evaluate the corresponding changes.