What is it about?
Microbial cells are optimized to increase the production of minicircles, a special type of DNA molecules that can be used in gene therapy applications. Specifically, the focus is on the modification of a pre-sequence of the gene that codes for par A resolvase, a key enzyme in the production of minicircles.
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Why is it important?
The improvement of the ability of microbial cells to produce minicircles will contribute to solve one of the bottlenecks that is currently hampering the use of minicircles in gene therapy applications, i.e. the lack of high-producer cell systems.
Perspectives
Minor changes in the 5′ untranslated region (5′-UTR) of parA messenger RNA, but a significant outcome in Par A resolvase and in minicrcle production.
Professor Duarte Miguel Prazeres
Instituto Superior Técnico
Read the Original
This page is a summary of: Improvement of DNA minicircle production by optimization of the secondary structure of the 5′-UTR of ParA resolvase, Applied Microbiology and Biotechnology, May 2016, Springer Science + Business Media,
DOI: 10.1007/s00253-016-7565-x.
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