What is it about?
In order to select plant cells and calluses for their resistance to phytotoxic compounds produced by pathogenic fungi, we developed a method of associated cultures of plant and fungus, which is an adaptation of the double layer technique classically used in microbiology. This method was applied to the selection of sugar beet or wheat calluses for their resistance to Cercospora beticola or Septoria nodorum, respectively. The fungal cultures was covered with culture medium containing a fungistatic concentration of mycostatin or Benlate. After diffusion of metabolic compounds from the fungal culture, calluses were transplanted on top of the second medium layer.
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Why is it important?
The system produced different metabolites according to the medium compostion of the fungal mycelium and could be used to investigate the connection between the state of the fungal metbolism and its toxic activity on plant calluses used as a biotest.
Perspectives
In particular it was observed that when previously growing calluses imbeded in the medium that would support the mycelium growth, the presence of plant compatible tissues elicited a complitely different fungal behaviour. This extended version of the double-layer culture system in principle allows to study the tripple relationship between plant elecitors, fungal metabolism, and fungal toxicity.
Nicolas Carels
Oswaldo Cruz Foundation
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This page is a summary of: Double-Layer Culture Technique as a Tool for the Selection of Calluses Resistant to Toxic Material from Plant Pathogenic Fungi, January 1986, Springer Science + Business Media,
DOI: 10.1007/978-94-015-7733-5_6.
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