Kinetic of protochlorophyllide photoreduction in situ

What is it about?

Dark-grown plants accumulate protochlorophyllide, a chlorophyll precursor, under several protein-photoenzyme complexes. Depending of the nature of the cofactor, NADPH or NADP+, the complexes are photoactive or nonphotoactive. Photoactive means that the photoenzyme is able to transform protochlorophyllide to chlorophyllide under a short (<2ms). As several types of photoactive photoactive complexes exist in vivo, we wanted to determine how the individual type of complexes contribute to the kinetic

Featured Image

Why is it important?

Absorbance and fluorescence kinetics of photoactive protochlorophyllide photoreduction were recorded simultaneously recorded for the first time at room temperature. The could be modelized using a monoexponential law, suggesting that the different types that the different types of photoactive protochlorophyllide are photoreduced at the same speed at room temperature