What is it about?
Delivery of genetic material to specific tissues, cells, and subcellular localisations is a major issue facing the progress of non-viral gene delivery. In this paper we investigate poly-L-lysine dendrons combined with cell penetrating peptides as a means to improve the delivery properties of plasmid DNA.
Why is it important?
Gene therapy is a potentially exciting way to treat or cure various diseases. However, the delivery of genes to their required site of action, without degradation has proven difficult. This work investigates various poly-L-lysine dendrons in combination with cell penetrating peptides in terms of: complexation with DNA; the size and charge of complexes; cellular toxicity; and cellular uptake. This information is important for the development of new non-viral gene delivery systems.
The following have contributed to this page: Dr Peter Michael Moyle