Peptide-Based Multicomponent Oligonucleotide Delivery Systems: Optimisation of Poly-l-lysine Dendrons for Plasmid DNA Delivery

Khairul A. Kamaruzaman, Peter M. Moyle, Istvan Toth
  • International Journal of Peptide Research and Therapeutics, July 2016, Springer Science + Business Media
  • DOI: 10.1007/s10989-016-9545-5

What is it about?

Delivery of genetic material to specific tissues, cells, and subcellular localisations is a major issue facing the progress of non-viral gene delivery. In this paper we investigate poly-L-lysine dendrons combined with cell penetrating peptides as a means to improve the delivery properties of plasmid DNA.

Why is it important?

Gene therapy is a potentially exciting way to treat or cure various diseases. However, the delivery of genes to their required site of action, without degradation has proven difficult. This work investigates various poly-L-lysine dendrons in combination with cell penetrating peptides in terms of: complexation with DNA; the size and charge of complexes; cellular toxicity; and cellular uptake. This information is important for the development of new non-viral gene delivery systems.

Perspectives

Dr Peter Michael Moyle (Author)
University of Queensland

This paper provides a detailed study of the ease of synthesis of different sized poly-L-lysine dendrons, and characterises these different species in terms of their capacity to delivery plasmid DNA into cells. This information is very important for optimising the use of poly-L-lysine dendrons as non-viral gene delivery platforms.

The following have contributed to this page: Dr Peter Michael Moyle