New single-step marker swap cassettes for fission yeast

What is it about?

Construction of multiply mutated strains for genetic interaction analysis and of strains carrying different epitope tags at multiple open reading frames for testing protein localization, abundance and protein-protein interactions is hampered by the availability of a sufficient number of different selectable genetic markers. The constructs presented and validated in this article simplify the construction of multiply deleted and/or tagged fission yeast strains from already existing genetic modifications.

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Why is it important?

The marker swap cassettes presented in this article will simplify the construction of fission yeast strains deleted and/or tagged at multiple loci. These will be useful tools for the fission yeast community to swap deletions and tagged versions of open reading frames between widely used and novel genetic markers.