What is it about?
We report on developing a long-term, non-invasive, and minute-frequency 3D RI imaging system to explore the status of live suspension cells with a high spatiotemporal scale (e.g., 0.71 μm, 1 min). We constructed the first 3D RI spatiotemporal spectrum and observed cell fate at frequency-on-demand (e.g., 1-10 min intervals for more than 5 h), bridging the gap between long-time monitoring (e.g., >5 h) and rapid imaging (e.g., 0.5-5 min).
Featured Image
Photo by Polina Kondrashova on Unsplash
Why is it important?
The work is a breakthrough against previous 3D RI imaging devices/systems, which are dedicated to short-term observation, regardless of the status of cells.
Perspectives
Experiments revealed different characteristics during normal cell growth, drug-induced cell apoptosis, and necrosis of drug-treated cells. We believe that the imaging framework and biological observations reported in this work will open up new frontiers of visualizing intracellular structures and will find many biological and medical applications such as disease diagnosis and nanomedicine.
Professor Wenhui Wang
Tsinghua University
Read the Original
This page is a summary of: Non‐Invasive and Minute‐Frequency 3D Tomographic Imaging Enabling Long‐Term Spatiotemporal Observation of Single Cell Fate, Small Methods, March 2023, Wiley,
DOI: 10.1002/smtd.202201492.
You can read the full text:
Contributors
The following have contributed to this page
