What is it about?
We propose a camera-free intrinsic mechanical cytometry (CFIMC) for on-the-fly measurement of two major intrinsic mechanical parameters, i.e., Young’s modulus E and fluidity β, of single cells. The strategy of CFIMC adopts a framework that couples the impedance electrodes with the constriction channel spatially, so that the impedance signals contain the dynamic deformability information of the cell squeezing through the constriction channel.
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Why is it important?
Mechanical properties of single cells are important label-free biophysical biomarkers, but normally measured by expensive and complex imaging systems. We unlock this limit and allow mechanical properties comparable across different measurement platforms by extracting intrinsic parameters.
Perspectives
The CFIMS strategy offers a new platform for high-throughput and real-time single-cell intrinsic mechanical characterization, facilitating on-chip integration of multiple modules such as sorting for downstream analysis of target cells. We envision that CFIMS opens a new avenue for high-throughput and real-time single cell analysis like intrinsic mechanical characterization.
Professor Wenhui Wang
Tsinghua University
Read the Original
This page is a summary of: Impedance‐Enabled Camera‐Free Intrinsic Mechanical Cytometry, Small Methods, May 2022, Wiley,
DOI: 10.1002/smtd.202200325.
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