What is it about?
A biomolecular recognition approach to functionalize cellulose with gold nanoparticles is described that relies on a complex of a fusion protein and an antibody.
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Why is it important?
Using the new approach, biomolecular recognition events are signaled by red color. This opens up the way for the development of simple and straightforward paper/cellulose-based tests where detection of a target analyte can be made by direct use of color signaling.
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This page is a summary of: A biomolecular recognition approach for the functionalization of cellulose with gold nanoparticles, Journal of Molecular Recognition, April 2017, Wiley,
DOI: 10.1002/jmr.2634.
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Resources
Gold nanoparticles anchored on cellulose by biomolecular recognition
Scanning electron microscopy analysis of the distribution of 40 nm biotin-gold nanoparticles immobilized on chromatographic paper (Whatman N. 1) modified with a complex of 2 recognition elements: a ZZ-CBM3 fusion that combines a carbohydrate-binding module (CBM) with the ZZ fragment of the staphylococcal protein A and an anti-biotin antibody. Nanoparticle density is approximately 100 AuNPs per square micrometer. Scale bar is 100 nm.
Gold nanoparticles anchored on cellulose microparticles by biomolecular recognition
Scanning electron microscopy analysis of the distribution of 40 nm biotin-gold nanoparticles immobilized on cellulose microparticles (Sigmacell 20) modified with a complex of 2 recognition elements: a ZZ-CBM3 fusion that combines a carbohydrate-binding module (CBM) with the ZZ fragment of the staphylococcal protein A and an anti-biotin antibody. Scale bar is 100 nm.
Biorecognition as a tool for the functionalization of cellulose
A brief overview of my research on the use of carbohydrate binding modules as a tool for the functionalization of cellulose-based materials.
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