Novel Dual Two-Dimensional Liquid Chromatography Online Coupled to Ultraviolet Detector, Fluorescence Detector, Ion-Trap Mass Spectrometer for Short Peptide Amino Acid Sequence Determination with Bottom-Up Strategy

What is it about?

Amino acid sequence in a peptide or protein is important to determine its three dimensional configuration structure, thus its biological function. We developed a multi-dimension liquid chromatography-mass spectrometry which can be employed to determine the primary structure (amino acid sequence) of a short peptide easily, quickly, and directly without result to a protein library. In addition, the D- or L-enantiomer of the amino acid can be determined.

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Why is it important?

This novel method can be used to establish new type of amino acid sequencer.

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