Determination of Isoproturon in Environmental Samples using QuEChERS Extraction‐Spectrofluorimetry

What is it about?

An accurate and sensitive method has been developed for determination of isoproturon herbicide using QuEChERS extraction‐spectrofluorimetric technique. The method involves the reaction of 2‐Cyanoacetamide with isoproturon in basic medium (NH3; 15 mol/L). The resulting fluorescent product was found to show maximum emission at 378 nm and maximum excitation at 333 nm. Fluorescence intensity under the influence of different parameters was investigated. The linear range of analyte's concentration was found to be 0.5 – 15 µg/mL with limit of detection 0.144 µg/mL and limit of quantification 0.437 µg/mL (S/N=3) and regression coefficient of 0.9991, under the optimized conditions. The proposed method was effectively applied for determination of isoproturon in different matrices with percent recoveries varying in the range 85.00 ± 1.2% ‐ 96.00 ± 0.5%. The method was also applied for residue analysis of isoproturon in real soil samples collected from a pilot field. For extraction of isoproturon QuEChERS extraction approach was used and the average residue in the soil samples was found to be 0.81±0.07 µg/g. In order to show the potentiality of current method, the results obtained with the proposed method were compared with other methods reported in literature.

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