The role of the substrate in enzymatic dehydrogenations
What is it about?
Using calculations we show that an intrinsic molecular property (called "negative hyperconjugation") governs the oxidation chemistry of L-lactate and may help to determine its enzymatic dehydrogenation mechanism.
Why is it important?
Flavoenzymes are a class of enzymes implicated in fundamental reactions of cell metabolism. How do these enzymes work? The question is still open for the oxidation of L-lactate in flavocytochrome-b2. We found that the oxidation mechanism of L-lactate in this enzyme is governed by a specific property of the substrate. We found this property to be also present in the substrates of other flavoenzymes, for which the mechanism was known. This validates and generalizes our findings. Hence, calculations on substrate molecules can give us precious information on the inner workings of an important class of enzymes.
The following have contributed to this page: Gloria Tabacchi
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