What is it about?
Development of sustainable biobutanol production platforms from lignocellulosic materials is impeded by inefficient five carbon sugar uptake by solventogenic bacteria. The recently isolated Clostridium sp. strain BOH3 is particularly advantaged in this regard as it serves as a model organism which can simultaneously utilize both glucose and xylose for high butanol (>15 g/L) production. Strain BOH3 was, therefore, investigated for its metabolic mechanisms for efficient five carbon sugar uptake using an 8-plex iTRAQ based quantitative proteomics approach.
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Why is it important?
Through this study we have elucidated uptake mechanisms of five and six carbon sugars for a given WT Clostridial strain. The study lays the platform for metabolic engineering strategies in designing organisms for efficient butanol and other value-added chemicals such as riboflavin production.
Perspectives
Development of sustainable biobutanol production platforms from lignocellulosic materials is impeded by inefficient five carbon sugar uptake by solventogenic bacteria. Hence this article is interesting particularly in two aspects: On the one hand here we talk about a novel Clostridial strain which can almost equally utilize both five and six carbon sugars, (a rarely observed phenomenon) which is further advantaged by the significant riboflavin titre levels achievable. On the other hand we have tried to utilize state of the art LC-MS based quantitative proteomics approach to reveal the important molecular machineries involved in achieving such a feat by the organism under consideration. This article therefore sheds light into various genetic targets that can be used for engineering organisms capable of large-scale production of butanol and other value-added products.
Anindya Basu
Rajiv Gandhi Proudyogiki Vishwavidyalaya
Read the Original
This page is a summary of: Quantitative proteome profiles help reveal efficient xylose utilization mechanisms in solventogenic Clostridium
sp. strain BOH3, Biotechnology and Bioengineering, May 2017, Wiley,
DOI: 10.1002/bit.26332.
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