What is it about?
Here, we propose an impedance-based electrical-mechanical flow cytometry framework for on-the-fly five-dimensional intrinsic measurement, i.e., radius r, cytoplasm conductivity σi, specific membrane capacitance Csm, Young’s modulus E, and fluidity β, of single cells.
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Photo by Kai Dahms on Unsplash
Why is it important?
With multimodal high-dimensional characterization, the electrical-mechanical flow cytometry can better reveal the difference of cell types, demonstrated by the experimental results with three types of cancer cells (HepG2, MCF-7 and MDA-MB-468) with 93.4% classification accuracy and pharmacological perturbations of the cytoskeleton (fixed and Cytochalasin B treated cells) with 95.1% classification accuracy, which is markedly higher than those based on single-modal electrical or mechanical parameters.
Perspectives
Without needing a camera for deformation monitoring, this cytometry stands as a first-of-its-kind high-throughput, high-efficiency cell characterization and phenotyping platform for multi-modal parameters.
Professor Wenhui Wang
Tsinghua University
Read the Original
This page is a summary of: Impedance‐Based Multimodal Electrical‐Mechanical Intrinsic Flow Cytometry, Small, July 2023, Wiley,
DOI: 10.1002/smll.202303416.
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