Super-resolution deep imaging with hollow Bessel beam STED microscopy

  • Super-resolution deep imaging with GB-STED microscopy
  • Wentao Yu, Ziheng Ji, Dashan Dong, Xusan Yang, Yunfeng Xiao, Qihuang Gong, Peng Xi, Kebin Shi
  • Laser & Photonics Review, November 2015, Wiley
  • DOI: 10.1002/lpor.201500151

Super-resolution, deep imaging with GB-STED

What is it about?

Although STED super-resolution provides new level of insight to biology, it is limited to 2-D due to the scattering of the specimen. By modulating the STED beam to a hollow Bessel beam, we demonstrate 3D deep imaging with Gaussian-Bessel STED (GB-STED), reaching 150 um depth with constant super-resolution.

Why is it important?

Life takes the form of 3D. Yet, despite of previous super-resolution microscopy providing us more indepth understanding to the subcellular activities, it is often limited in 2D, with shallow imaging depth. Our GB-STED can yield deep 3D super-resolution for specimen with large scattering such as brain, enabling the super-resolution in tissue level.


Dr Peng Xi
Peking University

By using the self-healing capability of Bessel beam in STED, we have realized deep 3-D STED super-resolution, on specimen with large refractive index mismatch, and high scattering coefficient. Comparing with the adjustment of correction collar for deep 3D imaging, GB-STED is much faster, without human intervene, and itmaintains its super-resolution for much deeper imaging.

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The following have contributed to this page: Dr Peng Xi and Xusan Yang